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Distortion was also observed in samples containing lipids, particularly in the Hoki roe homogenate. Pokhariyal et al. [49] described the complexity of protein bands inside the SDS-PAGE was increased during the existence of lipid, which brought about distortion of protein bands.
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This supports the rivalry that these pretreatments have an impact on the protein framework and substrate atmosphere, thus lessening hydrolysis a lot more than when just one pretreatment is used.
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